Plasticity of the histamine H 3 receptors after acute vestibular lesion in the adult cat
authors
keywords
- Histamine H 3 receptor
- Unilateral vestibular neurectomy
- Vestibular compensation
- Vestibular nuclei
- Tuberomammillary nuclei
- Inferior olive
- Solitary nucleus
- Cat
document type
ARTabstract
After unilateral vestibular neurectomy (UVN) many molecular and neurochemical mechanisms underlie the neurophysiological reorganizations occurring in the vestibular nuclei (VN) complex, as well as the behavioral recovery process. As a key regulator, the histaminergic system appears to be a likely candidate because drugs interfering with histamine (HA) neurotransmission facilitate behavioral recovery after vestibular lesion. This study aimed at analyzing the post-lesion changes of the histaminergic system by quantifying binding to histamine H 3 receptors (H 3 R; mediating namely histamine autoinhibition) using a histamine H 3 3 receptor agonist ([ H]N-α-methylhistamine). Experiments were done in brain sections of control cats (N = 6) and cats submitted to UVN and killed 1 (N = 6) or 3 (N = 6) weeks after the lesion. UVN induced a bilateral decrease in binding density of the agonist [ 3 H]N-α-methylhistamine to H 3 R in the tuberomammillary nuclei (TMN) at 1 week post-lesion, with a predominant down-regulation in the ipsilateral TMN. The bilateral decrease remained at the 3 weeks survival time and became symmetric. Concerning brainstem structures, binding density in the VN, the prepositus hypoglossi, the subdivisions of the inferior olive decreased unilaterally on the ipsilateral side at 1 week and bilaterally 3 weeks after UVN. Similar changes were observed in the subdivisions of the solitary nucleus only 1 week after the lesion. These findings indicate vestibular lesion induces plasticity of the histamine H 3 R, which could contribute to vestibular function recovery.
